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AMPK beta (PRKAB1) (N-Term), (AA 4-34) antibody

Details for Product No. ABIN391054
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Antigen
Epitope
N-Term, AA 4-34
Reactivity
Human, Mouse (Murine)
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN391054
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Immunogen This AMPK beta (PRKAB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 AA from the N-terminal region of human AMPK beta (PRKAB1).
Clone RB3228
Isotype Ig
Specificity This PRKAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB1.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Synonyms: 5'-AMP-activated protein kinase, beta-1 subunit, AMPK beta-1 chain, AMPKb
Molecular Weight 30251 DA
Gene ID 5564
UniProt NP_006244
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-AMPK beta (PRKAB1) (N-Term), (AA 4-34) antibody The anti-PRKAB1 Pab (ABIN391054) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).
anti-AMPK beta (PRKAB1) (N-Term), (AA 4-34) antibody (2) Western blot analysis of anti-PRKAB1 Antibody (N-term) (ABIN391054) in mouse heart lysates (35 µg/lane). PRKAB1 (arrow) was detected using the purified polyclonal antibody.
anti-AMPK beta (PRKAB1) (N-Term), (AA 4-34) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Landree, Hanlon, Strong et al.: "C75, a fatty acid synthase inhibitor, modulates AMP-activated protein kinase to alter neuronal energy metabolism." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3817-27, 2004 (PubMed).

Andersson, Filipsson, Abbott et al.: "AMP-activated protein kinase plays a role in the control of food intake." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12005-8, 2004 (PubMed).

Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).

Validation Images
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