Deoxycytidine Kinase (DCK) (C-Term), (AA 171-200) antibody

Details for Product No. ABIN391125
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Antigen
Synonyms dck, DCK, Dck, dck2, wu:fe11h12, wu:fj05e11, zgc:109951, wu:fc15f06, zgc:101771, dck1
Epitope
C-Term, AA 171-200
(10), (9), (8), (6), (4), (4), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(77), (26), (21), (12), (2), (2), (1), (1), (1)
Host
Rabbit
(51), (26)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP)
(60), (22), (13), (13), (13), (12), (11), (10), (8), (6), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN391125
291.50 $
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Immunogen This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 AA from the C-terminal region of human DCK.
Clone RB05276
Isotype Ig
Specificity This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the C-terminal region of human DCK.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name DCK
Background Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.
Synonyms: Deoxycytidine kinase, dCK
Molecular Weight 30518 DA
Gene ID 1633
UniProt P27707
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure
Application Notes IHC = 1:10-50, IF = 1:10-50, WB = 1:1000, IP = 1:500-1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Deoxycytidine Kinase (DCK) (C-Term), (AA 171-200) antibody The anti-DCK Pab (ABIN391125) is used in Western blot to detect DCK in mouse intestine tissue lysate.
anti-Deoxycytidine Kinase (DCK) (C-Term), (AA 171-200) antibody (2) Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (ABIN391125) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (ABIN391125). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
anti-Deoxycytidine Kinase (DCK) (C-Term), (AA 171-200) antibody (3) DCK Antibody (C-term) (ABIN391125)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Background publications Chottiner, Shewach, Datta et al.: "Cloning and expression of human deoxycytidine kinase cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 4, pp. 1531-5, 1991 (PubMed).

General Hartford, Duan, Delaney et al.: "Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity." in: Blood, Vol. 113, Issue 10, pp. 2145-53, 2009 (PubMed).

Validation Images
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