Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (C-Term), (AA 361-390) antibody

Details for Product No. ABIN391394
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Antigen
Synonyms CAL, FIG, PIST, GOPC1, dJ94G16.2, fe38f01, wu:fe38f01, zgc:56254, zgc:65853, 2210402P09Rik, AI844555, cal, gopc1, pist, DKFZp459H2130
Epitope
C-Term, AA 361-390
(17), (6), (5), (3), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(64), (41), (31), (17), (15), (12), (12), (1), (1)
Host
Rabbit
(64), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(56), (28), (26), (15), (10), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN391394
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 AA from the C-terminal region of human PIST.
Clone RB13073
Isotype Ig
Specificity This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368~398 amino acids from the C-terminal region of human PIST.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PIST
Background PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Synonyms: PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma
Molecular Weight 50389 DA
Gene ID 57120
UniProt Q9HD26
Research Area Protein Modifications, Autophagy, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (C-Term), (AA 361-390) antibody Western blot analysis of PIST Antibody (C-term) (ABIN391394) in Ramos cell line lysates (35 µg/lane). PIST (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC) (C-Term), (AA 361-390) antibody (2) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Wente, Stroh, Beaudet et al.: "Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5." in: The Journal of biological chemistry, Vol. 280, Issue 37, pp. 32419-25, 2005 (PubMed).

Hosts (64), (6)
Reactivities (64), (41), (31), (17), (15), (12), (12), (1), (1)
Applications (56), (28), (26), (15), (10), (2), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (6), (5), (3), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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