Nemo-Like Kinase (NLK) (C-Term), (AA 406-436) antibody

Details for Product No. ABIN391786
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Antigen
Epitope
C-Term, AA 406-436
(17), (12), (7), (7), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(63), (34), (26), (24), (24), (12)
Host
Rabbit
(62), (7)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(50), (23), (20), (19), (11), (8), (7), (3), (1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN391786
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Immunogen This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 AA from the C-terminal region of human NLK.
Clone RB3358
Isotype Ig
Specificity This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402~432 amino acids from the C-terminal region of human NLK.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NLK
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Synonyms: nemo-like kinase
Molecular Weight 57048 DA
Gene ID 51701
NCBI Accession NP_057315
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nemo-Like Kinase (NLK) (C-Term), (AA 406-436) antibody Western blot analysis of anti-NLK Pab (ABIN391786) in A375 cell lysate. NLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Nemo-Like Kinase (NLK) (C-Term), (AA 406-436) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Brott, Pinsky, Erikson: "Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 3, pp. 963-8, 1998 (PubMed).

Kehrer-Sawatzki, Moschgath, Maier et al.: "Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts." in: Gene, Vol. 251, Issue 1, pp. 63-71, 2000 (PubMed).

Ishitani, Kishida, Hyodo-Miura et al.: "The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 131-9, 2002 (PubMed).

Ishitani, Ninomiya-Tsuji, Matsumoto: "Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1379-89, 2003 (PubMed).

Yasuda, Tsuchiya, Yamada et al.: "Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells." in: Biochemical and biophysical research communications, Vol. 308, Issue 2, pp. 227-33, 2003 (PubMed).

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