Protein Kinase, CGMP-Dependent, Type II (PRKG2) (C-Term), (AA 714-744) antibody

Details for Product No. ABIN392537
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Antigen
Synonyms cGKII, PRKGR2, AW212535, CGKII, Prkgr2, cGK-II, GDPKII, MGC82580, PRKG2, si:dkey-18e17.2
Epitope
C-Term, AA 714-744
(5), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(27), (19), (19)
Host
Rabbit
(27)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(24), (22), (4), (2)
Pubmed 4 references available
Catalog no. ABIN392537
Quantity 400 µL
Price
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Immunogen This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-744 AA from the C-terminal region of human cGKII.
Clone RB01036
Isotype Ig
Specificity This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717~746 amino acids from the C-terminal region of human cGKII.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name cGKII
Background CGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.
Synonyms: cGMP-dependent protein kinase 2, Type II cGMP-dependent protein kinase, PRKGR2, PRKG2
Molecular Weight 87432 DA
Gene ID 5593
UniProt Q13237
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.71 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Protein Kinase, CGMP-Dependent, Type II (PRKG2) (C-Term), (AA 714-744) antibody Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (ABIN392537). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).
anti-Protein Kinase, CGMP-Dependent, Type II (PRKG2) (C-Term), (AA 714-744) antibody (2) Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (ABIN392537) in MC3T3 lysate.
anti-Protein Kinase, CGMP-Dependent, Type II (PRKG2) (C-Term), (AA 714-744) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Fujii, Ogata, Takahashi et al.: "Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells." in: FEBS letters, Vol. 375, Issue 3, pp. 263-7, 1996 (PubMed).

Orstavik, Solberg, Taskén et al.: "Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase." in: Biochemical and biophysical research communications, Vol. 220, Issue 3, pp. 759-65, 1996 (PubMed).

Gambaryan, Palmetshofer, Glazova et al.: "Inhibition of cGMP-dependent protein kinase II by its own splice isoform." in: Biochemical and biophysical research communications, Vol. 293, Issue 5, pp. 1438-44, 2002 (PubMed).

Vaandrager, Hogema, Edixhoven et al.: "Autophosphorylation of cGMP-dependent protein kinase type II." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28651-8, 2003 (PubMed).

Hosts (27)
Reactivities (27), (19), (19)
Applications (24), (22), (4), (2)
Epitopes (5), (3), (3), (2), (2), (1), (1), (1), (1)
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