Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody

Details for Product No. ABIN392631
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Antigen
Synonyms dgki, E130307B02Rik, ENSMUSG00000075035, F730209L11Rik, mDGK[z], DAGK5, DAGK6, DGK-ZETA, hDGKzeta
Epitope
C-Term, AA 989-1019
(9), (6), (4), (3), (1), (1), (1), (1), (1)
Reactivity
Human
(28)
Host
Rabbit
(25), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(19), (18), (12), (9), (8), (4), (3), (2), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN392631
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Immunogen This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 AA from the C-terminal region of human DGKZ.
Clone RB3716
Isotype Ig
Specificity This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 997~1026 amino acids from the C-terminal region of human DGKZ.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DGKZ
Background DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.
Synonyms: Diacylglycerol kinase, zeta, Diglyceride kinase, DGK-zeta, DAG kinase zeta, DAGK6
Pathways: Glycerophospholipid metabolism, Glycerolipid metabolism, Phosphatidylinositol signaling system
Molecular Weight 124122 Da
Gene ID 8525
UniProt Q13574
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody (2) Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) (ABIN392631). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Bunting, Tang, Zimmerman et al.: "Molecular cloning and characterization of a novel human diacylglycerol kinase zeta." in: The Journal of biological chemistry, Vol. 271, Issue 17, pp. 10230-6, 1996 (PubMed).

Ding, Bunting, Topham et al.: "Alternative splicing of the human diacylglycerol kinase zeta gene in muscle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5519-24, 1997 (PubMed).

Topham, Bunting, Zimmerman et al.: "Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta." in: Nature, Vol. 394, Issue 6694, pp. 697-700, 1998 (PubMed).

Hogan, Shepherd, Chabot et al.: "Interaction of gamma 1-syntrophin with diacylglycerol kinase-zeta. Regulation of nuclear localization by PDZ interactions." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26526-33, 2001 (PubMed).

Validation Images
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