Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody

Details for Product No. ABIN392631
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Synonyms DAGK5, DAGK6, DGK-ZETA, hDGKzeta, E130307B02Rik, ENSMUSG00000075035, F730209L11Rik, mDGK[z], dgki
C-Term, AA 989-1019
(4), (3), (2), (1), (1), (1), (1)
(17), (2), (1)
Clonality (Clone)
Polyclonal ()
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(12), (11), (8), (5), (4), (2), (2), (2), (1)
Pubmed 4 references available
Catalog no. ABIN392631
Quantity 400 µL
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Immunogen This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 AA from the C-terminal region of human DGKZ.
Clone RB3716
Isotype Ig
Specificity This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 997~1026 amino acids from the C-terminal region of human DGKZ.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DGKZ
Background DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.
Synonyms: Diacylglycerol kinase, zeta, Diglyceride kinase, DGK-zeta, DAG kinase zeta, DAGK6
Pathways: Glycerophospholipid metabolism, Glycerolipid metabolism, Phosphatidylinositol signaling system
Molecular Weight 124122 Da
Gene ID 8525
UniProt Q13574
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) (ABIN392631). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Diacylglycerol Kinase, zeta (DGKZ) (C-Term), (AA 989-1019) antibody (3) SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
Background publications Bunting, Tang, Zimmerman et al.: "Molecular cloning and characterization of a novel human diacylglycerol kinase zeta." in: The Journal of biological chemistry, Vol. 271, Issue 17, pp. 10230-6, 1996 (PubMed).

Ding, Bunting, Topham et al.: "Alternative splicing of the human diacylglycerol kinase zeta gene in muscle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5519-24, 1997 (PubMed).

Topham, Bunting, Zimmerman et al.: "Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta." in: Nature, Vol. 394, Issue 6694, pp. 697-700, 1998 (PubMed).

Hogan, Shepherd, Chabot et al.: "Interaction of gamma 1-syntrophin with diacylglycerol kinase-zeta. Regulation of nuclear localization by PDZ interactions." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26526-33, 2001 (PubMed).

Hosts (17), (2), (1)
Reactivities (20)
Applications (12), (11), (8), (5), (4), (2), (2), (2), (1)
Epitopes (4), (3), (2), (1), (1), (1), (1)
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