NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 350-382), (Center) antibody

Details for Product No. ABIN392649
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Antigen
Epitope
AA 350-382, Center
(32), (8), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(83), (19), (17), (12), (12), (12)
Host
Rabbit
(66), (15), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(73), (50), (10), (10), (10), (7), (4), (2), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN392649
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Immunogen This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-382 AA from the Central region of human NEK2.
Clone RB2995
Isotype Ig
Specificity This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359~388 amino acids from the center region of human NEK2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NEK2
Background NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers. This antibody is specific to isoform 2 of NEK2.
Synonyms: Serine threonine protein kinase Nek2, HsPK 21, HsPK21, NEK 2, NEK 2A, NEK2A, Never in mitosis gene A related kinase 2, NIMA (never in mitosis gene a) related kinase 2, NimA like protein kinase 1, NIMA related kinase 2, NimA related protein kinase 2, NLK 1
Molecular Weight 44905 DA
Gene ID 4751
UniProt Q96QN9
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 350-382), (Center) antibody The anti-NEK2 Pab (ABIN392649) is used in Western blot to detect NEK2 in HeLa cell lysate.
Background publications Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

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