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Details for Product No. ABIN400798

Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody

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Antigen
Synonyms H731, Ma3, Tis, D19Ucla1, Dug, h731, MGC69337, PDCD4
Epitope
»Alternatives pSer457
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN400798
Quantity 25 µL
Price
280.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Clone 9G6
Isotype IgG1
Characteristics Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like
Purification Protein A Chromatography
Alternative Name PDCD4
Background Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits thehelicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of thetranslation initiation complex. Pdcd4 also suppresses the transactivation of activatorprotein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylationsites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causesnuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 tointerfere with the transactivation of AP-1-responsive promoters by c-Jun.
Gene ID 27250
UniProt Q53EL6
Research Area Cancer, Apoptosis/Necrosis, Transcription Factors, Signaling
Application Notes ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 µg/ml, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, 0.01% Sodium Azide as preservative
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25µl) dilute 1: 10 by adding 225 µl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, 2005 (PubMed).

Palamarchuk, Efanov, Maximov et al.: "Akt phosphorylates and regulates Pdcd4 tumor suppressor protein." in: Cancer research, Vol. 65, Issue 24, pp. 11282-6, 2005 (PubMed).

Huang, Wu, Loeb et al.: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, 2009 (PubMed).

Alternatives for antigen "Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4)", type "Antibodies"
Hosts (97), (19), (4)
Reactivities (125), (75), (74), (12), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (106), (75), (39), (34), (11), (10), (10), (5), (3), (3), (2), (2)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (20), (19), (9), (9), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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