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Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody

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Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
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Clonality (Clone)
Monoclonal ()
(7), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(191), (121), (89), (41), (16), (11), (9), (8), (6), (2), (2), (1)
Pubmed 3 references available
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Quantity 25 μL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Clone 9G6
Isotype IgG1
Specificity This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat and Xenopus (100 % ).
Species reactivity (tested):Human.
Characteristics Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like
Purification Protein A Chromatography
Alternative Name PDCD4 (PDCD4 Antibody Abstract)
Background Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4
Gene ID 27250
UniProt Q53EL6
Research Area Cancer, Apoptosis/Necrosis, Transcription Factors, Signaling
Application Notes ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody (ABIN400798) anti-Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody
Immunohistochemistry (IHC) image for anti-Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody (ABIN400798) anti-Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) (pSer457) antibody (Image 2)
Background publications Huang, Wu, Loeb et al.: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, 2009 (PubMed).

Palamarchuk, Efanov, Maximov et al.: "Akt phosphorylates and regulates Pdcd4 tumor suppressor protein." in: Cancer research, Vol. 65, Issue 24, pp. 11282-6, 2005 (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, 2005 (PubMed).

Catalog No. ABIN400798
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