SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody

Details for Product No. ABIN401032
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Antigen
Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Reactivity
Cow (Bovine), Dog (Canine), Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
(84), (26), (23), (6), (6), (2)
Host
Rabbit
(70), (10), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(78), (56), (25), (20), (11), (10), (3), (2), (2)
Pubmed 4 references available
Quantity 0.5 mg
Shipping to United States (Change)
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Catalog No. ABIN401032
390.50 $
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Immunogen Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST
Isotype IgG
Specificity This antibody is directed against SUMO Activating Enzyme E1 protein.
Purification Affinity chromatography on Protein A
Alternative Name SAE1 / AOS1
Background SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin- like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the Nterminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.
Alternate names: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
Gene ID 10055
NCBI Accession NP_005491
UniProt Q9UBE0
Application Notes ELISA: 1/5,000 - 1/20,000. Western Blot: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.1 mL of deionized water or equivalent.
Concentration 5.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Lois, Lima: "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1." in: The EMBO journal, Vol. 24, Issue 3, pp. 439-51, 2005 (PubMed).

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