Tubulin, alpha 1B (TUBA1B) (AA 427-441) antibody

Details for Product No. ABIN401310
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Antigen
Synonyms K-ALPHA-1, k-alpha-1, tuba, tuba1, Tuba2, RGD1565476, TUBA3, TUBAT
Epitope
AA 427-441
(5), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity
Bird (Avian), Human, Mammalian
(29), (13), (12), (7), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1)
Host
Rabbit
(33), (14), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (2), (1), (1)
Application
ELISA, Western Blotting (WB)
(43), (28), (22), (21), (21), (13), (6), (5), (3), (1)
Pubmed 2 references available
Quantity 0.2 mg
Shipping to United States (Change)
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Catalog No. ABIN401310
379.50 $
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Immunogen Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin
Isotype IgG
Specificity This antibody is directed against alpha Tubulin protein. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent Loading Control.
Purification Immunoaffinity Chromatography.
Alternative Name alpha Tubulin / TUBA1B
Background Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post- translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
Alternate names: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha- ubiquitous chain
Gene ID 10376
NCBI Accession NP_006073
UniProt P68363
Application Notes ELISA: 1/5,000-1/25,000. Immunofluorescence: 1/500-1/2,000Western Blot: 1/500-1/3,000. Expect a band at ~50 kDa in size corresponding to alpha tubulin by Western blotting inmost cell lysates or extracts. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.08 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Supplier Images
anti-Tubulin, alpha 1B (TUBA1B) (AA 427-441) antibody anti-Tubulin, alpha 1B (TUBA1B) (AA 427-441) antibody
Background publications Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, 1985 (PubMed).

Cowan, Dobner, Fuchs et al.: "Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions." in: Molecular and cellular biology, Vol. 3, Issue 10, pp. 1738-45, 1984 (PubMed).

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