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Details for Product No. ABIN401558

Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibody

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Antigen
Synonyms 80K-L, MACS, PKCSL, PRKCSL, Macs, KINC, macs, marcks, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, MGC89127, MARCKS
Epitope
»Alternatives pSer170
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN401558
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-Sp-F-K).
Specificity This antibody detects endogenous levels of MARCKS only when phosphorylated at Threonine 170.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MARCKS
Background MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
Alternate names: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
Gene ID 4082
NCBI Accession NP_002347
UniProt P29966
Research Area Cardiovascular
Application Notes Western blot: 1/500~1/1000. Immunohistochemistry on Paraffin Sections: 1/50~1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
Background publications Hartwig, Thelen, Rosen et al.: "MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin." in: Nature, Vol. 356, Issue 6370, pp. 618-22, 1992 (PubMed).

Thelen, Rosen, Nairn et al.: "Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane." in: Nature, Vol. 351, Issue 6324, pp. 320-2, 1991 (PubMed).

Graff, Young, Johnson et al.: "Phosphorylation-regulated calmodulin binding to a prominent cellular substrate for protein kinase C." in: The Journal of biological chemistry, Vol. 264, Issue 36, pp. 21818-23, 1990 (PubMed).

Ramsden: "MARCKS: a case of molecular exaptation?" in: The international journal of biochemistry & cell biology, Vol. 32, Issue 5, pp. 475-9, 2001 (PubMed).

Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
Hosts (188), (10), (2)
Reactivities (176), (134), (125), (53), (40), (36), (24), (4), (3), (1), (1), (1)
Applications (139), (54), (48), (40), (29), (21), (16), (5), (4), (2), (1), (1)
Conjugates (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (28), (26), (23), (21), (21), (9), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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