Ras Homolog Gene Family, Member T1 (RHOT1) (Middle Region) antibody

Details for Product No. ABIN405705
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Antigen
Synonyms rhot1, zgc:55581, zgc:77063, wu:fd14e11, wu:fi14a05, arht2, miro-2, rasl, ARHT1, MIRO-1, MIRO1, 2210403N23Rik, AA415293, AF244542, AI834919, Arht1, C430039G08Rik, Miro1, miro1, si:dkeyp-97g3.6
Epitope
Middle Region
(14), (7), (7), (3), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Zebrafish (Danio rerio), Xenopus laevis, Human, Chicken
(30), (8), (6), (3), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(18), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(30), (16), (3), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN405705
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human RHOT1
Sequence ASAVTVTRDK KIDLQKKQTQ RNVFRCNVIG VKNCGKSGVL QALLGRNLMR
Predicted Reactivity Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 90 %, African clawed frog : 80 %
Characteristics This is a rabbit polyclonal antibody against RHOT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RHOT1
Background Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Molecular Weight 71 kDa
Gene ID 55288
NCBI Accession NP_060777, NM_018307
UniProt Q8IXI2
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 618 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Ras Homolog Gene Family, Member T1 (RHOT1) (Middle Region) antibody WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate
Background publications Fransson, Ruusala, Aspenström: "The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking." in: Biochemical and biophysical research communications, Vol. 344, Issue 2, pp. 500-10, 2006 (PubMed).

Validation Images
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