ELF3 antibody (Middle Region)

Catalog No. ABIN486862

Product Details anti-ELF3 Antibody

Target: ELF3 (E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))

Binding Specificity: Middle Region

Reactivity: Human, Cow, Dog, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GSCGAGAPSP GSSDVSTAGT GASRSSHSSD SGGSDVDLDP TDGKLFPSDG

Predicted Reactivity: Cow: 86%, Dog: 79%, Horse: 92%, Human: 100%, Pig: 93%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against ELF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human Elf3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 371 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ELF3

Target: ELF3 (E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))

Alternative Name: Elf3 (ELF3 Products)

Synonyms: ert antibody, esx antibody, epr-1 antibody, ese-1 antibody, ESE-1 antibody, ESX antibody, jen antibody, EPR-1 antibody, ERT antibody, E74 like ETS transcription factor 3 antibody, E74-like factor 3 antibody, elf3 antibody, Elf3 antibody, ELF3 antibody

Background: ELF3 is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family. ELF3 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ELF3 is expressed exclusively in epithelial cells. ELF3 is expected to be a critical regulator of epithelial cell differentiation. ETS factor ELF3 is a transcriptional regulator of angiopoietin-1 gene regulation in the setting of inflammation. ELF3 overexpressed early during human breast tumorigenesis. ELF3 expression confers a transformed and in vitro metastatic phenotype to otherwise normal MCF-12A cells. ELF3 mediates the expression of TGF-beta RII, and the transcriptional inhibition of ets-related transcription factor could be a one of the mechanisms of colonic carcinogenesis. ELF3 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP.
Alias Symbols: EPR-1, ERT, ESE-1, ESX
Protein Interaction Partner: MED23, XRCC5, RPL6, XRCC6, EP300, CBR3, Jun, MYC, BTRC, UBC, PAK1, TAF9, SUMO2, TBP, EPS15, CREBBP, NFKB1, EGFR, ERBB2, EHF, GANAB, ISG15, TARS, P4HB, KRT82, KRT31, JAK1, EEF1D, DDX3X, CALR, ATIC,
Protein Size: 371

Molecular Weight: 41 kDa

Gene ID: 1999

NCBI Accession: NM_004433, NP_004424

UniProt: P78545