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Beclin 1, Autophagy Related (BECN1) (N-Term) antibody

Details for Product No. ABIN497971
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Antigen
Synonyms beclin-1, atg6, vps30, beclin1, Beclin-1, becn1, zgc:73097, ATG6, VPS30, 4921513J16Rik, 5430417M23Rik, Atg6
Epitope
N-Term
(23), (13), (9), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(210), (85), (58), (32), (30), (21), (18), (15), (12), (6), (2), (1), (1)
Host
Rabbit
(161), (56), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (9), (9), (9), (8), (8), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(185), (84), (69), (53), (39), (31), (16), (16), (16), (10), (5), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN497971
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Immunogen Beclin-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of Human Beclin-1.
Isotype IgG
Specificity Recognizes Beclin-1 (N-term).
Purification Immunoaffinity Chromatography
Alternative Name Beclin-1
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).
Alternate names: BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197
Gene ID 8678
NCBI Accession NP_003757
UniProt Q14457
Application Notes ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 0.5–1 μg/ml. Positive Control: 293 Cell Lysate. Immunohistochemistry on Paraffin Sections. Positive Control: Rat Brain Tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8°C. Antibodies should not be exposed to prolonged high temperatures.
Expiry Date 12 months
Background publications Kisen, Tessitore, Costelli et al.: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994 (PubMed).

Kametaka, Okano, Ohsumi et al.: "Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 273, Issue 35, pp. 22284-91, 1998 (PubMed).

Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004 (PubMed).

Pattingre, Tassa, Qu et al.: "Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy." in: Cell, Vol. 122, Issue 6, pp. 927-39, 2005 (PubMed).

Validation Images
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