TTC8 antibody (Internal Region)

Catalog No. ABIN498031

This Goat Polyclonal antibody specifically detects TTC8 in WB and EIA. It exhibits reactivity toward Human, Mouse, Cow, Dog and Pig.

Quick Overview for TTC8 antibody (Internal Region) (ABIN498031)

Target: TTC8 (Tetratricopeptide Repeat Domain 8 (TTC8))

Reactivity: Human, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This TTC8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TTC8 Antibody

Binding Specificity: Internal Region

Sequence: C-KEVLKQDNTH VE

Specificity: This antibody is expected to recognize all reported isoforms (NP_653197.2, NP_938051.1, NP_938052.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog), Bovine and Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_653197.2, NP_938051.1, NP_938052.1.

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.2-0.6 μg/mL. Approx 60 kDa band observed in Human Testis lysates(calculated MW of 58.0 kDa according to NP_653197.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TTC8

Target: TTC8 (Tetratricopeptide Repeat Domain 8 (TTC8))

Alternative Name: TTC8 / BBS8

Background: Defects in TTC8 are the cause of Bardet-Biedl syndrome type 8 (BBS8). This is a genetically heterogeneous, autosomal recessive disorder and the primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. Alternate transcriptional splice variants have been characterized.Synonyms: Bardet-Biedl syndrome 8 protein, TPR repeat protein 8, Tetratricopeptide repeat protein 8

Gene ID: 123016

NCBI Accession: NP_653197

UniProt: Q8TAM2

Pathways: Hedgehog Signaling