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ANAPC4 antibody (Anaphase Promoting Complex Subunit 4) (N-Term)

Details for Product anti-ANAPC4 Antibody No. ABIN499297, Supplier: Login to see
Antigen
  • MGC81278
  • DDBDRAFT_0186409
  • DDBDRAFT_0234268
  • DDB_0186409
  • DDB_0234268
  • APC4
  • ANAPC4
  • 2610306D21Rik
  • D5Ertd249e
  • F18E5.150
  • F18E5_150
Epitope
N-Term
15
13
12
10
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
48
16
15
Host
Rabbit
46
2
Clonality
Polyclonal
Conjugate
This ANAPC4 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
43
31
16
4
3
1
1
1
Supplier
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Immunogen APC4 antibody was raised against a 14 amino acid peptide near the amino terminus of human APC4.
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name APC4 / ANAPC4 (ANAPC4 Antibody Abstract)
Background Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.Synonyms: Anaphase-promoting complex subunit 4, Cyclosome subunit 4
Gene ID 29945
UniProt Q9UJX5
Pathways
Application Notes ELISA. Western Blot: APC4 antibody can be used for detection of APC4 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-ANAPC4 antibody (Anaphase Promoting Complex Subunit 4) (N-Term) (ABIN499297) Western blot analysis of APC4 in mouse liver tissue lysate with APC4 antibody at (A) ...
Background publications Peters: "The anaphase promoting complex/cyclosome: a machine designed to destroy." in: Nature reviews. Molecular cell biology, Vol. 7, Issue 9, pp. 644-56, 2006 (PubMed).

Thornton, Ng, Matyskiela et al.: "An architectural map of the anaphase-promoting complex." in: Genes & development, Vol. 20, Issue 4, pp. 449-60, 2006 (PubMed).

Jörgensen, Gräslund, Betz et al.: "Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex." in: Gene, Vol. 262, Issue 1-2, pp. 51-9, 2001 (PubMed).