Anaphase Promoting Complex Subunit 4 (ANAPC4) (N-Term) antibody

Details for Product No. ABIN499297
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms MGC81278, DDBDRAFT_0186409, DDBDRAFT_0234268, DDB_0186409, DDB_0234268, APC4, ANAPC4, 2610306D21Rik, D5Ertd249e, F18E5.150, F18E5_150
Epitope
N-Term
(9), (7), (6), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(29), (11), (11)
Host
Rabbit
(27), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
(28), (19), (10), (3), (3), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN499297
401.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen APC4 antibody was raised against a 14 amino acid peptide near the amino terminus of human APC4.
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name APC4 / ANAPC4
Background Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.Synonyms: Anaphase-promoting complex subunit 4, Cyclosome subunit 4
Gene ID 29945
UniProt Q9UJX5
Application Notes ELISA. Western Blot: APC4 antibody can be used for detection of APC4 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Background publications Jörgensen, Gräslund, Betz et al.: "Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex." in: Gene, Vol. 262, Issue 1-2, pp. 51-9, 2001 (PubMed).

Thornton, Ng, Matyskiela et al.: "An architectural map of the anaphase-promoting complex." in: Genes & development, Vol. 20, Issue 4, pp. 449-60, 2006 (PubMed).

Peters: "The anaphase promoting complex/cyclosome: a machine designed to destroy." in: Nature reviews. Molecular cell biology, Vol. 7, Issue 9, pp. 644-56, 2006 (PubMed).

Validation Images
Did you look for something else?
back to top