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Acyl-CoA Binding Domain Containing 3 (Acbd3) (N-Term) antibody

Details for Product No. ABIN499900, Supplier: Log in to see
Antigen
  • fa20g08
  • si:dkey-267p15.1
  • wu:fa20g08
  • zgc:66303
  • cb366
  • GOLPH1
  • sb:cb366
  • wu:fa04b12
  • wu:fd14b03
  • wu:fi29h06
  • ACBD3
  • acbd3
  • MGC122176
  • GCP60
  • GOCAP1
  • PAP7
  • T22A6.60
  • T22A6_60
  • acyl-CoA-binding domain 3
  • 60kDa
  • 8430407O11Rik
  • D1Ertd10e
  • Gocap1
  • Pap7
Epitope
N-Term
9
5
5
5
4
2
1
1
Reactivity
Human
59
25
9
2
2
Host
Rabbit
34
26
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
48
19
11
11
10
10
9
2
Supplier
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Immunogen GOLPH1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human GOLPH1.
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name ACBD3 (Acbd3 Antibody Abstract)
Background GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.Synonyms: Acyl-CoA-binding domain-containing protein 3, GOCAP1, GOLPH1, Golgi complex-associated protein 1, Golgi phosphoprotein 1, Golgi resident protein GCP60, PAP7, PBR- and PKA-associated protein 7, Peripheral benzodiazepine receptor-associated protein
Gene ID 64746
NCBI Accession NP_073572
UniProt Q9H3P7
Research Area Signaling, Organelles
Pathways
Application Notes ELISA. Western Blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Acyl-CoA Binding Domain Containing 3 (Acbd3) (N-Term) antibody (ABIN499900) Western blot analysis of GOLPH1 in K562 cell lysate with AP30370PU-N GOLPH1 antibody ...
Background publications Sbodio, Machamer: "Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with golgin-160." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 29874-81, 2007 (PubMed).

Zhou, Atkins, Rompani et al.: "The mammalian Golgi regulates numb signaling in asymmetric cell division by releasing ACBD3 during mitosis." in: Cell, Vol. 129, Issue 1, pp. 163-78, 2007 (PubMed).

Sohda, Misumi, Yamamoto et al.: "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45298-306, 2001 (PubMed).

Mancini, Machamer, Roy et al.: "Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis." in: The Journal of cell biology, Vol. 149, Issue 3, pp. 603-12, 2000 (PubMed).