IRGM antibody (Center)

Catalog No. ABIN500070

Product Details anti-IRGM Antibody

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects IRGM at center.

Purification: Peptide affinity chromatography

Immunogen: IRGM antibody was raised against a 14 amino acid peptide near the center of the human IRGM.

Isotype: IgG

Application Details

Application Notes: ELISA: Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for IRGM

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Alternative Name: IRGM / LRG-47 (IRGM Products)

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.Synonyms: IFI1, IRGM1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1, Interferon-inducible protein 1, LRG47

Gene ID: 345611

UniProt: F8VPC9