Engrailed Homeobox 1 (EN1) (Middle Region) antibody

Details for Product No. ABIN501632
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Antigen
Synonyms EN1, En-1, Mo-en.1, ENG-1, en-1, en1, eng1, zgc:100771, en1-A, engrailed-1, xen1, Gg-En-1, en-3
Epitope
Middle Region
(8), (6), (2), (1), (1), (1)
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(24), (1)
Host
Rabbit
(14), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(24), (19), (14), (5), (3), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human EN1
Sequence LNESQIKIWF QNKRAKIKKA TGIKNGLALH LMAQGLYNHS TTTVQDKDES
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Pig : 100 %, Rabbit : 100 %, Chicken : 90 %, African clawed frog : 80 %
Characteristics This is a rabbit polyclonal antibody against EN1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name EN1 (EN1 Antibody Abstract)
Background Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Differe
Molecular Weight 40 kDa
Gene ID 2019
NCBI Accession NM_001426, NP_001417
UniProt Q05925
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 392 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Engrailed Homeobox 1 (EN1) (Middle Region) antibody WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
Background publications Atit, Sgaier, Mohamed et al.: "Beta-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouse." in: Developmental biology, Vol. 296, Issue 1, pp. 164-76, 2006 (PubMed).

Validation Images
Catalog No. ABIN501632
289.00 $
Plus shipping costs $45.00
Quantity
Price
50 μg
289.00 $

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