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SH3 Domain Containing Ring Finger 1 (SH3RF1) (Middle Region) antibody

Details for Product No. ABIN502370, Supplier: Login to see New
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Synonyms SH3MD2, posh, xPOSH, rnf142, sh3md2, MGC135632, MGC189605, POSH, RNF142, 2200003J05Rik, Posh, R75531, Sh3md2, si:ch211-114c9.2, si:dkey-15j16.4, wu:fi42f11
Middle Region
(3), (3), (2), (1), (1), (1), (1), (1)
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(31), (20), (19)
(25), (6)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(20), (11), (8), (6), (4)
Pubmed 1 reference available
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Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Synthetic peptide directed towards the middle region of human SH3RF1
Blocking Peptide Blocking peptide for this product available: ABIN983699
Predicted Reactivity Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Dog : 90 %, Pig : 90 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against SH3RF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SH3RF1 (SH3RF1 Antibody Abstract)
Background SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.
Molecular Weight 93 kDa
Gene ID 57630
NCBI Accession NM_020870, NP_065921
UniProt Q7Z6J0
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 888 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) (Middle Region) antibody (ABIN502370) WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Lyons, Thorburn, Ryan et al.: "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21987-97, 2007 (PubMed).

Catalog No. ABIN502370
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