SH3 Domain Containing Ring Finger 1 (SH3RF1) (Middle Region) antibody

Details for Product No. ABIN502370
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Antigen
Synonyms SH3MD2, posh, xPOSH, rnf142, sh3md2, MGC135632, MGC189605, POSH, RNF142, 2200003J05Rik, Posh, R75531, Sh3md2, si:ch211-114c9.2, si:dkey-15j16.4, wu:fi42f11
Epitope
Middle Region
(7), (3), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Xenopus laevis, Human
(31), (17), (16), (12), (12), (12)
Host
Rabbit
(20), (11)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (13), (10), (4), (4)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502370
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human SH3RF1
Sequence LLKLLSGAST KRKPRVSPPA SPTLEVELGS AELPLQGAVG PELPPGGGHG
Predicted Reactivity Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Dog : 90 %, Pig : 90 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against SH3RF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SH3RF1
Background SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.
Molecular Weight 93 kDa
Gene ID 57630
NCBI Accession NM_020870, NP_065921
UniProt Q7Z6J0
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 888 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) (Middle Region) antibody WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145 cell lysate
Background publications Lyons, Thorburn, Ryan et al.: "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21987-97, 2007 (PubMed).

Validation Images
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