ULP1 (ULP1) (N-Term) antibody

Details for Product No. ABIN503683
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Antigen
Synonyms NIB1, AA408656, Smt3ip, Smt3ip1, SMT3IP1, SSP3, Ulp1
Epitope
N-Term
(14), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Human
(44), (22), (14), (12), (12), (4), (1)
Host
Rabbit
(51), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(35), (28), (18), (10), (10), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN503683
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human SENP3
Sequence PPPKPRLKSG GGFGPDPGSG TTVPARRLPV PRPSFDASAS EEEEEEEEEE
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against SENP3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SENP3
Background SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
Molecular Weight 65 kDa
Gene ID 26168
NCBI Accession NP_056485, NM_015670
UniProt Q9H4L4
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 574 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-ULP1 (ULP1) (N-Term) antibody WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate
anti-ULP1 (ULP1) (N-Term) antibody (2) Sample Type: 1. Rat Liver cells (200ug)
2. Rat Brain cells (200ug)
3. Rat Testis cells (200ug)
4. Rat Kidney cells (200ug)
Primary Dilution: 1:1000
Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit
Secondary Dilution: 1:1000
Image Submitted by: Andreia Carvalho
IBMC-OBF, Portugal .

anti-ULP1 (ULP1) (N-Term) antibody (3) Sample Type: 1. HeLa cell extract (200ug)
Primary Dilution: 1:1000
Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit
Secondary Dilution: 1:1000
Image Submitted by: Andreia Carvalho
IBMC-OBF, Portugal .

anti-ULP1 (ULP1) (N-Term) antibody (4) WB Suggested Anti-SENP3
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500
Positive Control:.21_B cell lysate
SENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Background publications Haindl, Harasim, Eick et al.: "The nucleolar SUMO-specific protease SENP3 reverses SUMO modification of nucleophosmin and is required for rRNA processing." in: EMBO reports, Vol. 9, Issue 3, pp. 273-9, 2008 (PubMed).

Validation Images
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