WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1) (Middle Region) antibody

Details for Product No. ABIN504170
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Antigen
Synonyms zgc:64205, WIPI1, atg18, wipi49, MGC145296, ATG18, ATG18A, WIPI49, 4930533H01Rik, AW411817, D11Ertd498e, RGD1307754
Epitope
Middle Region
(7), (7), (7), (6), (5), (3), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Human
(45), (18), (18), (12)
Host
Rabbit
(35), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(36), (20), (10), (5), (4), (1)
Pubmed 1 reference available
Quantity 50 µg
Options
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Catalog No. ABIN504170
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human WIPI1
Sequence LLGSGTTEEN KENDLRPSLP QSYAATVARP SASSASTVPG YSEDGGALRG
Predicted Reactivity Dog : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Bovine : 90 %, Guinea pig : 90 %, Pig : 90 %, Rat : 90 %
Characteristics This is a rabbit polyclonal antibody against WIPI1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name WIPI1
Background WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI su
Molecular Weight 49 kDa
Gene ID 55062
NCBI Accession NM_017983, NP_060453
UniProt Q5MNZ9
Research Area Cancer
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 446 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1) (Middle Region) antibody WB Suggested Anti-WIPI1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate
anti-WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1) (Middle Region) antibody (2) Host: Rabbit
Target Name: WIPI1
Sample Tissue: Human Fetal Heart
Antibody Dilution: 1.0 µg/mL
anti-WD Repeat Domain, phosphoinositide Interacting 1 (WIPI1) (Middle Region) antibody (3) Host: Rabbit
Target Name: WIPI1
Sample Tissue: Human Fetal Lung
Antibody Dilution: 1.0 µg/mL
Background publications Proikas-Cezanne, Ruckerbauer, Stierhof et al.: "Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy." in: FEBS letters, Vol. 581, Issue 18, pp. 3396-404, 2007 (PubMed).

Validation Images
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