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RAD21 Homolog (S. Pombe) (RAD21) (AA 544-631), (C-Term) antibody

Details for Product No. ABIN567792, Supplier: Log in to see
Antigen
  • rad21
  • wu:fb55d02
  • wu:fb93f04
  • CDLS4
  • HR21
  • HRAD21
  • MCD1
  • NXP1
  • SCC1
  • hHR21
  • mKIAA0078
  • hr21
  • mcd1
  • nxp-1
  • nxp1
  • scc1
  • xrad21
Epitope
C-Term, AA 544-631
20
13
11
6
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
56
27
22
2
1
1
1
1
Host
Rat
48
10
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
46
12
11
10
9
8
6
4
2
2
1
1
Supplier
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Immunogen Recombinant C-terminal fragment (544-631 aa) of Scc1 subunit of Human Cohesin (hRad21).
Clone CM110-2C10
Isotype IgG1
Specificity This antibody reacts with Cohesin (120 kDa) on Western blotting.
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Alternative Name RAD21 (RAD21 Antibody Abstract)
Background Cohesin in eukaryotic cells is mediated by a multisubunit protein complex. Cohesin consists of four proteins: Smc1, Smc3, Scc1/Mcd1/hRad21, which is called kleisin and is the target of the protease separase, and Scc3/Irr1. Cohesin holds sister chromatids together until they separate into daughter cells during mitosis and also prevents splitting of chromosomes once bipolar attachments are made.Synonyms: Double-strand-break repair protein rad21 homolog, HR21, KIAA0078, NXP1, Nuclear matrix protein 1, SCC1 homolog
Gene ID 5885
NCBI Accession NP_006256
UniProt O60216
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-RAD21 Homolog (S. Pombe) (RAD21) (AA 544-631), (C-Term) antibody (ABIN567792) anti-RAD21 Homolog (S. Pombe) (RAD21) (AA 544-631), (C-Term) antibody
Western Blotting (WB) image for anti-RAD21 Homolog (S. Pombe) (RAD21) (AA 544-631), (C-Term) antibody (ABIN567792) anti-RAD21 Homolog (S. Pombe) (RAD21) (AA 544-631), (C-Term) antibody (Image 2)
Background publications Hoque, Ishikawa: "Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 5059-67, 2001 (PubMed).