Fatty Acid 2-Hydroxylase (FA2H) (AA 121-148), (Center) antibody

Details for Product No. ABIN650994
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Antigen
Epitope
AA 121-148, Center
(7), (3), (1)
Reactivity
Human, Mouse (Murine)
(17), (5), (2)
Host
Rabbit
(15), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(18), (8), (8), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN650994
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Immunogen This FA2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 AA from the Central region of human FA2H.
Clone RB23881
Isotype Ig
Specificity This FA2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127~157 amino acids from the Central region of human FA2H.
Predicted Reactivity Monkey,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name FA2H
Background FA2H is a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups.
Synonyms: Fatty acid alpha-hydroxylase, Fatty acid 2-hydroxylase,FAAH, FA2H
Molecular Weight 42791 DA
Gene ID 79152
UniProt Q7L5A8
Research Area Cell Signaling, Cell Structure, Metabolism, Neurology
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 121-148), (Center) antibody Western blot analysis of FA2H Antibody (Center) (ABIN650994) in ZR-75-1 cell line lysates (35 µg/lane). FA2H (arrow) was detected using the purified polyclonal antibody.
anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 121-148), (Center) antibody (2) Western blot analysis of FA2H Antibody (Center) (ABIN650994) in mouse brain tissue lysates (35 µg/lane). FA2H (arrow) was detected using the purified polyclonal antibody.
anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 121-148), (Center) antibody (3) FA2H Antibody (Center) (ABIN650994) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
Background publications Uchida, Hama, Alderson et al.: "Fatty acid 2-hydroxylase, encoded by FA2H, accounts for differentiation-associated increase in 2-OH ceramides during keratinocyte differentiation." in: The Journal of biological chemistry, Vol. 282, Issue 18, pp. 13211-9, 2007 (PubMed).

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