Zinc Finger, FYVE Domain Containing 16 (ZFYVE16) (N-Term), (AA 375-404) antibody

Details for Product No. ABIN651313
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Antigen
Synonyms RGD1564784, ZFYVE16, ENDOFIN, PPP1R69, AI035632, B130024H06Rik, B130031L15, mKIAA0305
Epitope
N-Term, AA 375-404
(7), (7), (3), (3), (1), (1)
Reactivity
Human
(22), (2), (2)
Host
Rabbit
(17), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(20), (16), (9), (8), (3), (2), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN651313
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Immunogen This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-404 AA from the N-terminal region of human ZFYVE16.
Clone RB23251
Isotype Ig
Specificity This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the N-terminal region of human ZFYVE16.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ZFYVE16
Background The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule.
Synonyms: Endosome-associated FYVE domain protein, Endofin, Zinc finger FYVE domain-containing protein 16,KIAA0305, ZFYVE16
Molecular Weight 168849 DA
Gene ID 9765
UniProt Q7Z3T8
Research Area Phospho-specific antibodies
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Zinc Finger, FYVE Domain Containing 16 (ZFYVE16) (N-Term), (AA 375-404) antibody Western blot analysis of ZFYVE16 Antibody (N-term) (ABIN651313) in K562 cell line lysates (35 µg/lane).ZFYVE16 (arrow) was detected using the purified polyclonal antibody.
anti-Zinc Finger, FYVE Domain Containing 16 (ZFYVE16) (N-Term), (AA 375-404) antibody (2) ZFYVE16 Antibody (N-term) (ABIN651313) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Zinc Finger, FYVE Domain Containing 16 (ZFYVE16) (N-Term), (AA 375-404) antibody (3) ZFYVE16 Antibody (N-term) (ABIN651313) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Shi, Chang, Nie et al.: "Endofin acts as a Smad anchor for receptor activation in BMP signaling." in: Journal of cell science, Vol. 120, Issue Pt 7, pp. 1216-24, 2007 (PubMed).

Chen, Low, Choong et al.: "Phosphoproteomics identified Endofin, DCBLD2, and KIAA0582 as novel tyrosine phosphorylation targets of EGF signaling and Iressa in human cancer cells." in: Proteomics, Vol. 7, Issue 14, pp. 2384-97, 2007 (PubMed).

Validation Images
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