Eukaryotic Translation Initiation Factor 3 Subunit G (EIF3G) (AA 209-238), (Center) antibody

Details for Product No. ABIN651535
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Antigen
Synonyms eIF3-S4, eIF3g-A, eif3s4-A, eif3s4, zgc:56553, EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44, 44kDa, D0Jmb4, Eif3s4, TU-189B2, p44, eif3s3
Epitope
AA 209-238, Center
(7), (7), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(29), (8), (8), (4), (3), (2), (2), (1), (1)
Host
Rabbit
(27), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(29), (15), (8), (8), (6), (4), (4), (2)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN651535
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Immunogen This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 AA from the Central region of human EIF3G.
Clone RB26846
Isotype Ig
Specificity This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human EIF3G.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name EIF3G
Background Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Synonyms: eIF3 p42, eIF3 p44, Eukaryotic translation initiation factor 3 RNA-binding subunit, eIF-3 RNA-binding subunit, eIF-3-delta, Eukaryotic translation initiation factor 3 subunit 4, Eukaryotic translation initiation factor 3 subunit G, eIF3g,EIF3S4, EIF3G
Molecular Weight 35611  DA
Gene ID 8666
NCBI Accession NP_003746
UniProt O75821
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Eukaryotic Translation Initiation Factor 3 Subunit G (EIF3G) (AA 209-238), (Center) antibody EIF3G Antibody (Center) (ABIN651535) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).
anti-Eukaryotic Translation Initiation Factor 3 Subunit G (EIF3G) (AA 209-238), (Center) antibody (2) EIF3G Antibody (Center) (ABIN651535) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Eukaryotic Translation Initiation Factor 3 Subunit G (EIF3G) (AA 209-238), (Center) antibody (3) EIF3G Antibody (Center) (ABIN651535) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Masutani, Sonenberg, Yokoyama et al.: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, 2007 (PubMed).

Zhou, Sandercock, Fraser et al.: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, 2008 (PubMed).

Validation Images
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