Aftiphilin (AFTPH) (C-Term), (AA 844-871) antibody

Details for Product No. ABIN651958
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Antigen
Synonyms RGD1311920, 9130023F12RIK, MGC151658, fb71f09, wu:fb71f09, CH73-131E21.7, si:ch73-131e21.7, MGC115640, Nbla10388, 9130023F12Rik, AU015099, AU016543, AU018432, AU019184, AU044244
Epitope
C-Term, AA 844-871
(7), (1), (1)
Reactivity
Human
(8)
Host
Rabbit
(8)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)
(8), (8), (7), (6), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN651958
291.50 $
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Immunogen This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 844-871 AA from the C-terminal region of human AFTPH.
Clone RB27293
Isotype Ig
Specificity This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 851-879 amino acids from the C-terminal region of human AFTPH.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name AFTPH
Background AFTPH may play a role in membrane trafficking.
Synonyms: Aftiphilin,AFTPH,AFTH
Molecular Weight 102200 DA
Gene ID 54812
NCBI Accession NP_001002243
UniProt Q6ULP2
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes IHC = 1:50-100, FACS = 1:10-50, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Aftiphilin (AFTPH) (C-Term), (AA 844-871) antibody AFTPH Antibody (C-term) (ABIN651958) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Aftiphilin (AFTPH) (C-Term), (AA 844-871) antibody (2) AFTPH Antibody (C-term) (ABIN651958) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Aftiphilin (AFTPH) (C-Term), (AA 844-871) antibody (3) AFTPH Antibody (C-term) (ABIN651958) western blot analysis in CEM,Jurkat,MCF-7 cell line lysates (35 µg/lane). This demonstrates the AFTPH antibody detected the AFTPH protein (arrow).
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Hirst, Borner, Harbour et al.: "The aftiphilin/p200/gamma-synergin complex." in: Molecular biology of the cell, Vol. 16, Issue 5, pp. 2554-65, 2005 (PubMed).

Burman, Wasiak, Ritter et al.: "Aftiphilin is a component of the clathrin machinery in neurons." in: FEBS letters, Vol. 579, Issue 10, pp. 2177-84, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Lui-Roberts, Ferraro, Nightingale et al.: "Aftiphilin and gamma-synergin are required for secretagogue sensitivity of Weibel-Palade bodies in endothelial cells." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5072-81, 2008 (PubMed).

Validation Images
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