EH-Domain Containing 3 (EHD3) (N-Term), (AA 22-48) antibody

Details for Product No. ABIN652345
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Antigen
Synonyms ehd3, h-past, hpast1, past, past1, EHD3, si:ch211-147c1.2, MGC146452, PAST3, Ehd2
Epitope
N-Term, AA 22-48
(7), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(21), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(12), (11)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(23), (13), (11), (7), (6), (5), (3), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN652345
291.50 $
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Immunogen This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 AA from the N-terminal region of human EHD3.
Clone RB17956
Isotype Ig
Specificity This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EHD3.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EHD3
Background EHD3 plays a role in endocytic transport.
Synonyms: PAST homolog 3, EH domain-containing protein 3,PAST3, EHD2, EHD3
Molecular Weight 61896 DA
Gene ID 30845
UniProt Q9NZN3
Research Area Signaling, Phospho-specific antibodies, Cell Structure, Protein Modifications, Cancer, Chromatin
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-EH-Domain Containing 3 (EHD3) (N-Term), (AA 22-48) antibody Western blot analysis of EHD3 Antibody (N-term) (ABIN652345) in mouse lung tissue lysates (35 µg/lane).EHD3 (arrow) was detected using the purified polyclonal antibody.
anti-EH-Domain Containing 3 (EHD3) (N-Term), (AA 22-48) antibody (2) EHD3 Antibody (N-term) (ABIN652345) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-EH-Domain Containing 3 (EHD3) (N-Term), (AA 22-48) antibody (3) EHD3 Antibody (N-term) (ABIN652345) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Roland, Kenworthy, Peranen et al.: "Myosin Vb interacts with Rab8a on a tubular network containing EHD1 and EHD3." in: Molecular biology of the cell, Vol. 18, Issue 8, pp. 2828-37, 2007 (PubMed).

Naslavsky, McKenzie, Altan-Bonnet et al.: "EHD3 regulates early-endosome-to-Golgi transport and preserves Golgi morphology." in: Journal of cell science, Vol. 122, Issue Pt 3, pp. 389-400, 2009 (PubMed).

Soranzo, Spector, Mangino et al.: "A genome-wide meta-analysis identifies 22 loci associated with eight hematological parameters in the HaemGen consortium." in: Nature genetics, Vol. 41, Issue 11, pp. 1182-90, 2009 (PubMed).

Validation Images
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