Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1) (N-Term), (AA 49-77) antibody

Details for Product No. ABIN652439
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Antigen
Synonyms CKIP-1, OC120, 2810052M02Rik, Ckip1, JZA-20, Jza2, RGD1311487
Epitope
N-Term, AA 49-77
(11), (4), (3), (1), (1)
Reactivity
Human
(32), (22), (22)
Host
Rabbit
(29), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(22), (18), (10), (9), (2), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN652439
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This CKIP-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-77 AA from the N-terminal region of human CKIP-1.
Clone RB19797
Isotype Ig
Specificity This CKIP-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~85 amino acids from the N-terminal region of human CKIP-1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CKIP-1
Background CKIP-1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). This protein may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). It appears to target ATM to the plasma membrane and appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
Synonyms: Pleckstrin homology domain-containing family O member 1, Casein kinase 2-interacting protein 1, CK2-interacting protein 1, CKIP-1, C-Jun-binding protein, JBP, Osteoclast maturation-associated gene 120 protein, HQ0024c, OC120, CKIP1, PLEKHO1
Molecular Weight 46237 DA
Gene ID 51177
UniProt Q53GL0
Research Area Cell Structure, Neurology
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1) (N-Term), (AA 49-77) antibody Western blot analysis of CKIP-1 Antibody (N-term) (ABIN652439) in 293 cell line lysates (35 µg/lane). CKIP-1 (arrow) was detected using the purified polyclonal antibody.
anti-Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1) (N-Term), (AA 49-77) antibody (2) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CKIP-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1) (N-Term), (AA 49-77) antibody (3) Flow cytometric analysis of 293 cells using CKIP-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Barrios-Rodiles, Brown, Ozdamar et al.: "High-throughput mapping of a dynamic signaling network in mammalian cells." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1621-5, 2005 (PubMed).

Hosts (29), (4)
Reactivities (32), (22), (22)
Applications (22), (18), (10), (9), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (4), (3), (1), (1)
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