phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 513-541), (Center) antibody

Details for Product No. ABIN652449
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Antigen
Synonyms Pla2c, cPLA2, Pla2g4, PLA2, PLA2G1B, CPLA2, PLA2G4, PLA2G4A, cPLA2alpha, cPLA2-alpha, pla2g4
Epitope
AA 513-541, Center
(16), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(54), (30), (28), (11), (11), (11)
Host
Rabbit
(49), (3), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(36), (23), (19), (16), (8), (8), (6), (4), (3), (2), (2)
Pubmed 2 references available
Catalog no. ABIN652449
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 AA from the Central region of human PLA2G4A.
Clone RB21320
Isotype Ig
Specificity This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520~549 amino acids from the Center region of human PLA2G4A.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus),Rabbit,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PLA2G4A
Background PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
Synonyms: Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4, CPLA2, PLA2G4A
Molecular Weight 85211 DA
Gene ID 5321
UniProt P47712
Research Area Neurology
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 513-541), (Center) antibody Western blot analysis of PLA2G4A Antibody (Center) (ABIN652449) in NCI-H460 cell line lysates (35 µg/lane). PLA2G4A (arrow) was detected using the purified polyclonal antibody.
anti-phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 513-541), (Center) antibody (2) Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 513-541), (Center) antibody (3) Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Sharp, White, Chiou et al.: "Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2." in: The Journal of biological chemistry, Vol. 266, Issue 23, pp. 14850-3, 1991 (PubMed).

Clark, Lin, Kriz et al.: "A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP." in: Cell, Vol. 65, Issue 6, pp. 1043-51, 1991 (PubMed).

Hosts (49), (3), (3)
Reactivities (54), (30), (28), (11), (11), (11)
Applications (36), (23), (19), (16), (8), (8), (6), (4), (3), (2), (2)
Conjugates (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (16), (5), (3), (1), (1), (1), (1), (1), (1), (1)
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