phosphoprotein Enriched in Astrocytes 15 (PEA15) (C-Term), (AA 86-115) antibody

Details for Product No. ABIN652463
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Antigen
Synonyms PEA15, DKFZp459O1410, HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED, Pea15a, Mat1, Pea15, Pkcs15
Epitope
C-Term, AA 86-115
(26), (20), (14), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(104), (74), (69), (36), (25), (3), (1)
Host
Rabbit
(102), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(58), (51), (35), (30), (15), (15), (3), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN652463
291.50 $
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Immunogen This PEA-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 AA from the C-terminal region of human PEA-15.
Clone RB21195
Isotype Ig
Specificity This PEA-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the C-terminal region of human PEA-15.
Predicted Reactivity Hamster,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PEA-15
Background PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.
Synonyms: Astrocytic phosphoprotein PEA-15, Phosphoprotein enriched in diabetes, PED, PEA15
Molecular Weight 15040 DA
Gene ID 8682
UniProt Q15121
Research Area Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (C-Term), (AA 86-115) antibody Western blot analysis of PEA-15 Antibody (C-term) (ABIN652463) in T47D cell line lysates (35 µg/lane).PEA-15 (arrow) was detected using the purified polyclonal antibody.
anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (C-Term), (AA 86-115) antibody (2) Western blot analysis of PEA-15 Antibody (C-term) (ABIN652463) in mouse lung tissue lysates (35 µg/lane).PEA-15 (arrow) was detected using the purified polyclonal antibody.
anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (C-Term), (AA 86-115) antibody (3) Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Product cited in: Lv, Ma, Zhang et al.: "Quantitative proteomics reveals that PEA15 regulates astroglial A? phagocytosis in an Alzheimer's disease mouse model." in: Journal of proteomics, Vol. 110C, pp. 45-58, 2014 (PubMed).

Background publications Trencia, Perfetti, Cassese et al.: "Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4511-21, 2003 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Validation Images
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