BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 116-143), (Center) antibody

Details for Product No. ABIN652514
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Antigen
Epitope
AA 116-143, Center
(11), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(24), (1), (1), (1), (1), (1)
Host
Rabbit
(20), (2), (1), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)
(21), (10), (8), (7), (4), (2), (2), (2)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN652514
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Immunogen This HSPC142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 AA from the Central region of human HSPC142.
Clone RB20468
Isotype Ig
Specificity This HSPC142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human HSPC142
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HSPC142
Background Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
Synonyms: BRCA1-A complex subunit MERIT40, Mediator of RAP80 interactions and targeting subunit of 40 kDa, New component of the BRCA1-A complex, HSPC142, NBA1, C19orf62, MERIT40
Molecular Weight 36560 DA
Gene ID 29086
UniProt Q9NWV8
Research Area Cancer, Neurology, Cell Structure
Application Notes IHC = 1:50-100, WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 116-143), (Center) antibody Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 116-143), (Center) antibody (2) Western blot analysis of HSPC142 Antibody (Center) (ABIN652514) in MDA-MB231 cell line lysates (35 µg/lane). HSPC142 (arrow) was detected using the purified polyclonal antibody.
anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (AA 116-143), (Center) antibody (3) Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Shao, Patterson-Fortin, Messick et al.: "MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks." in: Genes & development, Vol. 23, Issue 6, pp. 740-54, 2009 (PubMed).

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