Diaphanous Homolog 2 (Drosophila) (DIAPH2) (AA 870-899), (Center) antibody

Details for Product No. ABIN653356
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Antigen
Synonyms DIAPH2, MGC148327, DIA2, dia2, sb:eu1025, si:dkeyp-11a1.1, Dia3, Diaph2, Drf2, E430022I22Rik, DIA, DRF2, POF, POF2, Diap2, RGD1564399
Epitope
AA 870-899, Center
(7), (7), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(29), (12), (1), (1)
Host
Rabbit
(28), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(20), (10), (9), (7), (7), (6), (2), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN653356
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Immunogen This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 AA from the Central region of human DIAPH2.
Clone RB23866
Isotype Ig
Specificity This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877~907 amino acids from the Central region of human DIAPH2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DIAPH2
Background DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.
Synonyms: Diaphanous-related formin-2, DRF2, Protein diaphanous homolog 2, DIA, DIAPH2
Molecular Weight 125569 DA
Gene ID 1730
UniProt O60879
Research Area Signaling, Protein Modifications, Cancer, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Diaphanous Homolog 2 (Drosophila) (DIAPH2) (AA 870-899), (Center) antibody Western blot analysis of DIAPH2 Antibody (Center) (ABIN653356) in 293, MDA-MB435 cell line lysates (35 µg/lane). DIAPH2 (arrow) was detected using the purified polyclonal antibody.
anti-Diaphanous Homolog 2 (Drosophila) (DIAPH2) (AA 870-899), (Center) antibody (2) Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Diaphanous Homolog 2 (Drosophila) (DIAPH2) (AA 870-899), (Center) antibody (3) DIAPH2 Antibody (Center) (ABIN653356) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Satoh, Tominaga: "mDia-interacting protein acts downstream of Rho-mDia and modifies Src activation and stress fiber formation." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39290-4, 2001 (PubMed).

Gasman, Kalaidzidis, Zerial: "RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase." in: Nature cell biology, Vol. 5, Issue 3, pp. 195-204, 2003 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Validation Images
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