Complexin 1 (CPLX1) (AA 33-60), (Center) antibody

Details for Product No. ABIN653361
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Antigen
Epitope
AA 33-60, Center
(8), (6), (3), (1)
Reactivity
Human
(26), (15), (14), (12), (12), (12)
Host
Rabbit
(24), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(18), (10), (10), (7), (5), (1), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN653361
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Immunogen This CPLX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 AA from the Central region of human CPLX1.
Clone RB23961
Isotype Ig
Specificity This CPLX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~69 amino acids from the Central region of human CPLX1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Monkey,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CPLX1
Background CPLX1 encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin, part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it, allowing transmitter release.
Synonyms: Synaphin-2, Complexin I, CPX I, Complexin-1, CPLX1
Molecular Weight 15030 DA
Gene ID 10815
UniProt O14810
Research Area Neurology
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.32 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Complexin 1 (CPLX1) (AA 33-60), (Center) antibody Western blot analysis of CPLX1 Antibody (Center) (ABIN653361) in mouse brain tissue lysates (35 µg/lane). CPLX1 (arrow) was detected using the purified polyclonal antibody.
anti-Complexin 1 (CPLX1) (AA 33-60), (Center) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with CPLX1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Chen, Tomchick, Kovrigin et al.: "Three-dimensional structure of the complexin/SNARE complex." in: Neuron, Vol. 33, Issue 3, pp. 397-409, 2002 (PubMed).

Giraudo, Eng, Melia et al.: "A clamping mechanism involved in SNARE-dependent exocytosis." in: Science (New York, N.Y.), Vol. 313, Issue 5787, pp. 676-80, 2006 (PubMed).

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