Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) (N-Term), (AA 10-38) antibody

Details for Product No. ABIN653748
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Antigen
Synonyms CRTL1, Crtl1, LP, BB099155, CLP, Crtl1l, LP-1, crtl1, hapln1, wu:fc26f11, wu:fc37g04, wu:fc50e12, wu:fc55h01, CTRL1
Epitope
N-Term, AA 10-38
(4), (2), (2), (1)
Reactivity
Human
(8), (1), (1)
Host
Rabbit
(8), (1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(8), (3), (2), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN653748
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This HAPLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 AA from the N-terminal region of human HAPLN1.
Clone RB24639
Isotype Ig
Specificity This HAPLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human HAPLN1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HAPLN1
Background HAPLN1 belongs to the HAPLN family. It contains 1 Ig like V type (immunoglobulin like) domain and 2 Link domains. This protein stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Synonyms: Cartilage-linking protein 1, Cartilage-link protein, Proteoglycan link protein, Hyaluronan and proteoglycan link protein 1,CRTL1, HAPLN1
Molecular Weight 40166 Da
Gene ID 1404
UniProt P10915
Research Area Cancer, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) (N-Term), (AA 10-38) antibody HAPLN1 Antibody (N-term) (ABIN653748) western blot analysis in MCF-7,Jurkat,HepG2 cell line lysates (35 µg/lane).This demonstrates the HAPLN1 antibody detected the HAPLN1 protein (arrow).
anti-Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) (N-Term), (AA 10-38) antibody (2) HAPLN1 Antibody (N-term) (ABIN653748) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HAPLN1 Antibody (N-term)for immunohistochemistry.
anti-Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) (N-Term), (AA 10-38) antibody (3) HAPLN1 Antibody (N-term) (ABIN653748) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Seyfried, McVey, Almond et al.: "Expression and purification of functionally active hyaluronan-binding domains from human cartilage link protein, aggrecan and versican: formation of ternary complexes with defined hyaluronan oligosaccharides." in: The Journal of biological chemistry, Vol. 280, Issue 7, pp. 5435-48, 2005 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Hosts (8), (1)
Reactivities (8), (1), (1)
Applications (8), (3), (2), (2), (1), (1)
Epitopes (4), (2), (2), (1)
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