Nuclear Prelamin A Recognition Factor-Like (NARFL) (AA 240-269), (Center) antibody

Details for Product No. ABIN653781
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Antigen
Synonyms 19.m02295, HPRN, IOP1, LET1L, PRN, 9030612I22Rik, AI504405, im:7151348, zgc:158422
Epitope
AA 240-269, Center
(8), (6), (6), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(27), (3), (3)
Host
Rabbit
(27)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(27), (21), (9), (4)
Pubmed 3 references available
Quantity 400 µL
Options
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Catalog No. ABIN653781
291.50 $
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Immunogen This NARFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 AA from the Central region of human NARFL.
Clone RB22210
Isotype Ig
Specificity This NARFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247~277 amino acids from the Central region of human NARFL.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NARFL
Background Synonyms: Iron-only hydrogenase-like protein 1, IOP1, Protein related to Narf, Nuclear prelamin A recognition factor-like protein, Cytosolic Fe-S cluster assembly factor NARFL,PRN, NARFL
Molecular Weight 53020 DA
Gene ID 64428
UniProt Q9H6Q4
Research Area Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Nuclear Prelamin A Recognition Factor-Like (NARFL) (AA 240-269), (Center) antibody Western blot analysis of NARFL Antibody (Center) (ABIN653781) in MDA-MB435 cell line lysates (35 µg/lane). NARFL (arrow) was detected using the purified polyclonal antibody.
anti-Nuclear Prelamin A Recognition Factor-Like (NARFL) (AA 240-269), (Center) antibody (2) NARFL Antibody (Center) (ABIN653781) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Huang, Song, Flores et al.: "IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1alpha activity." in: The Biochemical journal, Vol. 401, Issue 1, pp. 341-52, 2006 (PubMed).

Song, Tu, Lee: "Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron-sulfur protein biogenesis." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35297-307, 2009 (PubMed).

Validation Images
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