DCP1 Decapping Enzyme Homolog B (S. Cerevisiae) (DCP1B) (AA 261-290), (Center) antibody

Details for Product No. ABIN653844
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Antigen
Epitope
AA 261-290, Center
(8), (6), (3), (1), (1), (1), (1)
Reactivity
Human
(18), (2), (2), (2), (2), (2)
Host
Rabbit
(17), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(16), (9), (8), (4), (2), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN653844
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Immunogen This DCP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 AA from the Central region of human DCP1B.
Clone RB24473
Isotype Ig
Specificity This DCP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268~298 amino acids from the Central region of human DCP1B.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DCP1B
Background DCP1B is a core component of the mRNA decapping complex, a key factor in the regulation of mRNA decay.
Synonyms: mRNA-decapping enzyme 1B,DCP1B
Molecular Weight 67851 DA
Gene ID 196513
UniProt Q8IZD4
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Metabolism, Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-DCP1 Decapping Enzyme Homolog B (S. Cerevisiae) (DCP1B) (AA 261-290), (Center) antibody Western blot analysis of DCP1B Antibody (center) (ABIN653844) in Ramos cell line lysates (35 µg/lane). DCP1B (arrow) was detected using the purified polyclonal antibody.
anti-DCP1 Decapping Enzyme Homolog B (S. Cerevisiae) (DCP1B) (AA 261-290), (Center) antibody (2) DCP1B Antibody (center) (ABIN653844) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

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