ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H) (AA 68-97), (Center) antibody

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Antigen
Synonyms ATPQ, 0610009D10Rik, Atp5jd, Atpq
Epitope
AA 68-97, Center
(7), (2), (1), (1), (1), (1)
Reactivity
Human
(20), (13), (10)
Host
Rabbit
(20)
Clonality (Clone)
Polyclonal ()
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)
(20), (12), (5), (3), (2), (1)
Pubmed 4 references available
Catalog no. ABIN654144
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 AA from the Central region of human ATP5H.
Clone RB23854
Isotype Ig
Specificity This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATP5H
Background Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Synonyms: ATP synthase subunit d, mitochondrial,ATP5H,
Molecular Weight 18491 DA
Gene ID 10476
UniProt NP_001003785.1, NP_006347.1, O75947
Research Area Signaling, Cell Signaling, Protein Modifications, Cell Structure
Application Notes IHC = 1:50-100, FACS = 1:10-50, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H) (AA 68-97), (Center) antibody ATP5H antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H) (AA 68-97), (Center) antibody (2) ATP5H Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H) (AA 68-97), (Center) antibody (3) ATP5H Antibody (Center) western blot analysis in HepG2,NCI-H460,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).
Background publications Oster, Wang: "Rotary protein motors." in: Trends in cell biology, Vol. 13, Issue 3, pp. 114-21, 2003 (PubMed).

Cross: "Molecular motors: turning the ATP motor." in: Nature, Vol. 427, Issue 6973, pp. 407-8, 2004 (PubMed).

Kim, Kim, Yoo et al.: "Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells." in: Cancer science, Vol. 99, Issue 10, pp. 1884-91, 2008 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).

Hosts (20)
Reactivities (20), (13), (10)
Applications (20), (12), (5), (3), (2), (1)
Epitopes (7), (2), (1), (1), (1), (1)
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