Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (AA 78-105), (Center) antibody

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Antigen
Synonyms gnat-3, Gtn, Ggust, GDCA
Epitope
AA 78-105, Center
(4), (2), (1)
Reactivity
Human
(18), (14), (14), (14), (12), (12)
Host
Rabbit
(14), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(10), (9), (8), (7), (2), (1)
Pubmed 2 references available
Catalog no. ABIN654441
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This GNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 AA from the Central region of human GNAT3.
Clone RB24621
Isotype Ig
Specificity This GNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human GNAT3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name GNAT3
Background Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase, Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
Synonyms: GNAT3,Guanine nucleotide-binding protein G(t) subunit alpha-3,
Molecular Weight 40357 DA
Gene ID 346562
UniProt A8MTJ3, NP_001095856.1
Research Area Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (AA 78-105), (Center) antibody GNAT3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GNAT3 antibody detected the GNAT3 protein (arrow).
anti-Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (AA 78-105), (Center) antibody (2) GNAT3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GNAT3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (AA 78-105), (Center) antibody (3) GNAT3 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Fehr, Meyer, Widmayer et al.: "Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa." in: Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, Vol. 193, Issue 1, pp. 21-34, 2007 (PubMed).

Jang, Kokrashvili, Theodorakis et al.: "Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 38, pp. 15069-74, 2007 (PubMed).

Hosts (14), (6)
Reactivities (18), (14), (14), (14), (12), (12)
Applications (10), (9), (8), (7), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (1)
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