GNAT3 antibody (AA 78-105)

Catalog No. ABIN654441

Product Details anti-GNAT3 Antibody

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Binding Specificity: AA 78-105

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAT3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the Central region of human GNAT3.

Clone: RB24621

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for GNAT3

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Alternative Name: GNAT3 (GNAT3 Products)

Synonyms: GDCA antibody, Ggust antibody, Gtn antibody, gnat-3 antibody, G protein subunit alpha transducin 3 antibody, guanine nucleotide binding protein, alpha transducing 3 antibody, GNAT3 antibody, Gnat3 antibody

Background: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase, Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

Molecular Weight: 40357

Gene ID: 346562

NCBI Accession: NP_001095856

UniProt: A8MTJ3

Pathways: Peptide Hormone Metabolism, G-protein mediated Events, Phototransduction