Asparaginyl-tRNA Synthetase (NARS) (N-Term), (AA 24-53) antibody

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Antigen
Synonyms
ASNRS, NARS1, C78150, AA960128, 3010001M15Rik, LRRGT00113, AsnRS, BcDNA:GH06451, CG10687, Ca, Dmel\\CG10687, NRS, l(2)37Ca, l(2)37Ca)E11, l(2)E11, fb57a10, wu:fb57a10, si:dkey-274m14.2, BA4802, RGD130 ... show more
ASNRS, NARS1, C78150, AA960128, 3010001M15Rik, LRRGT00113, AsnRS, BcDNA:GH06451, CG10687, Ca, Dmel\\CG10687, NRS, l(2)37Ca, l(2)37Ca)E11, l(2)E11, fb57a10, wu:fb57a10, si:dkey-274m14.2, BA4802, RGD1305145 show less
Epitope
N-Term, AA 24-53
(5), (3), (2), (2)
Reactivity
Human
(25), (6), (3)
Host
Rabbit
(15), (11)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(24), (11), (6), (5), (5), (3), (3), (2), (2)
Pubmed 3 references available
Catalog no. ABIN655041
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 AA from the N-terminal region of human NARS.
Clone RB14847
Isotype Ig
Specificity This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human NARS.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Monkey
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NARS
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
Synonyms: Asparaginyl-tRNA synthetase, cytoplasmic,NARS,
Molecular Weight 62943 DA
Gene ID 4677
UniProt NP_004530.1, O43776
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Asparaginyl-tRNA Synthetase (NARS) (N-Term), (AA 24-53) antibody Western blot analysis of NARS (arrow) using rabbit polyclonal NARS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.
anti-Asparaginyl-tRNA Synthetase (NARS) (N-Term), (AA 24-53) antibody (2) NARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Asparaginyl-tRNA Synthetase (NARS) (N-Term), (AA 24-53) antibody (3) NARS Antibody (N-term) western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).This demonstrates the NARS antibody detected the NARS protein (arrow).
anti-Asparaginyl-tRNA Synthetase (NARS) (N-Term), (AA 24-53) antibody (4) NARS Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Cirullo, Arredondo-Vega, Smith et al.: "Isolation and characterization of interspecific heat-resistant hybrids between a temperature-sensitive chinese hamster cell asparaginyl-tRNA synthetase mutant and normal human leukocytes: assignment of human asnS gene to chromosome 18." in: Somatic cell genetics, Vol. 9, Issue 2, pp. 215-33, 1983 (PubMed).

Beaulande, Tarbouriech, Härtlein: "Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence, functional expression in Escherichia coli and characterization as human autoantigen." in: Nucleic acids research, Vol. 26, Issue 2, pp. 521-4, 1998 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Hosts (15), (11)
Reactivities (25), (6), (3)
Applications (24), (11), (6), (5), (5), (3), (3), (2), (2)
Epitopes (5), (3), (2), (2)
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