APG7L-D555 (PEI 1:100)b (AA 540-569) antibody

Details for Product No. ABIN655142
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Antigen
Epitope
AA 540-569
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN655142
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Immunogen This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 AA from human APG7L.
Clone RB29229
Isotype Ig
Specificity This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-576 amino acids from human APG7L.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Synonyms: Ubiquitin-like modifier-activating enzyme ATG7,ATG7,APG7L
Molecular Weight 77960 DA
Gene ID 10533
NCBI Accession NP_006386, NP_001138384, NP_001129503
UniProt O95352
Research Area Signaling, Protein Modifications, Autophagy, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.41 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-APG7L-D555 (PEI 1:100)b (AA 540-569) antibody APG7L Antibody (ABIN655142) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Zhao, Yang, Liao et al.: "Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity." in: Nature cell biology, Vol. 12, Issue 7, pp. 665-75, 2010 (PubMed).

Metzger, Saukko, Van Che et al.: "Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7." in: Human genetics, Vol. 128, Issue 4, pp. 453-9, 2010 (PubMed).

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