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OPN-A/b (N-Term), (AA 14-40) antibody

Details for Product No. ABIN655514
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Antigen
Epitope
N-Term, AA 14-40
(1), (1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(1), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
Options
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Catalog No. ABIN655514
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 AA from the N-terminal region of human OPN-a/b.
Clone RB19160
Isotype Ig
Specificity This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-48 amino acids from the N-terminal region of human OPN-a/b.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
Synonyms: Osteopontin,SPP1,BNSP, OPN
Molecular Weight 35423 DA
Gene ID 6696
UniProt NP_000573.1, P10451, NP_001035147.1
Research Area Cell Structure, Neurology, Protein Modifications, Cell Signaling, Phospho-specific antibodies
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.28 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-OPN-A/b (N-Term), (AA 14-40) antibody Western blot analysis of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.
anti-OPN-A/b (N-Term), (AA 14-40) antibody (2) OPN-a/b Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OPN-a/b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-OPN-A/b (N-Term), (AA 14-40) antibody (3) Confocal immunofluorescent analysis of OPN-a/b Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Background publications Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN655514
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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