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Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody

Details for Product No. ABIN655643
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Antigen
Synonyms SSH3, BC028922, SSH-3, mSSH-3L, SSH3L
Epitope
C-Term, AA 575-602
(8), (8), (8), (7), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(40), (13), (12)
Host
Rabbit
(29), (11)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
(37), (28), (26), (13), (9), (8), (6), (6), (2)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN655643
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Immunogen This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 AA from the C-terminal region of human SSH3.
Clone RB19876
Isotype Ig
Specificity This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SSH3
Background The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1, MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Synonyms: RB19876
Pathways: Regulation of actin cytoskeleton
Molecular Weight 72996 Da
Gene ID 54961
UniProt Q8TE77
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.28 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (2) SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (3) SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (4) Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Background publications Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Kligys, Claiborne, DeBiase et al.: "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 32520-8, 2007 (PubMed).

Validation Images
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