Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody

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Synonyms SSH3L, SSH-3, mSSH-3L, BC028922, SSH3
C-Term, AA 575-602
(8), (2), (2), (2), (2), (1), (1), (1), (1)
(25), (13), (12)
(20), (5)
Clonality (Clone)
Polyclonal ()
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
(24), (16), (14), (6), (4), (4), (3), (2), (1)
Pubmed 2 references available
Catalog no. ABIN655643
Quantity 400 µL
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Immunogen This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 AA from the C-terminal region of human SSH3.
Clone RB19876
Isotype Ig
Specificity This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SSH3
Background The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1, MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Synonyms: RB19876
Pathways: Regulation of actin cytoskeleton
Molecular Weight 72996 Da
Gene ID 54961
UniProt Q8TE77
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.28 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody SSH3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow).
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (2) SSH3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (3) SSH3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term), (AA 575-602) antibody (4) Confocal immunofluorescent analysis of SSH3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Background publications Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Kligys, Claiborne, DeBiase et al.: "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 32520-8, 2007 (PubMed).

Hosts (20), (5)
Reactivities (25), (13), (12)
Applications (24), (16), (14), (6), (4), (4), (3), (2), (1)
Epitopes (8), (2), (2), (2), (2), (1), (1), (1), (1)
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