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phospholipase C-Like 2 (PLCL2) (C-Term), (AA 1099-1126) antibody

Details for Product No. ABIN655943
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Antigen
Epitope
C-Term, AA 1099-1126
(8), (7), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(17), (2), (2)
Host
Rabbit
(13), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(15), (12), (8), (7), (6), (4), (3), (2), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN655943
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Immunogen This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1099-1126 AA from the C-terminal region of human PLCL2.
Clone RB31611
Isotype Ig
Specificity This PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1100-1127 amino acids from the C-terminal region of human PLCL2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PLCL2
Background PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).
Synonyms: PLCL2,Inactive phospholipase C-like protein 2,KIAA1092, PLCE2
Molecular Weight 125866 DA
Gene ID 23228
UniProt NP_055999.2, Q9UPR0
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-phospholipase C-Like 2 (PLCL2) (C-Term), (AA 1099-1126) antibody PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).
anti-phospholipase C-Like 2 (PLCL2) (C-Term), (AA 1099-1126) antibody (2) PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-phospholipase C-Like 2 (PLCL2) (C-Term), (AA 1099-1126) antibody (3) Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
anti-phospholipase C-Like 2 (PLCL2) (C-Term), (AA 1099-1126) antibody (4) PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Background publications Nakayama, Kikuno, Ohara: "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs." in: Genome research, Vol. 12, Issue 11, pp. 1773-84, 2002 (PubMed).

Colland, Jacq, Trouplin et al.: "Functional proteomics mapping of a human signaling pathway." in: Genome research, Vol. 14, Issue 7, pp. 1324-32, 2004 (PubMed).

Raychaudhuri, Thomson, Remmers et al.: "Genetic variants at CD28, PRDM1 and CD2/CD58 are associated with rheumatoid arthritis risk." in: Nature genetics, Vol. 41, Issue 12, pp. 1313-8, 2009 (PubMed).

Validation Images
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