Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8) (C-Term), (AA 519-548) antibody

Details for Product No. ABIN656431
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Antigen
Epitope
C-Term, AA 519-548
(10), (9), (8), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(44), (20), (5), (4), (2), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(43), (4), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(48), (23), (8), (8), (7), (5), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN656431
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Immunogen This CCT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 AA from the C-terminal region of human CCT8.
Clone RB31130
Isotype Ig
Specificity This CCT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-548 amino acids from the C-terminal region of human CCT8.
Predicted Reactivity Mouse (Murine),Monkey
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CCT8
Background Molecular chaperone, CCT8 assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Synonyms: T-complex protein 1 subunit theta,CCT8,C21orf112, CCTQ, KIAA0002
Molecular Weight 59621 DA
Gene ID 10694
NCBI Accession NP_006576
UniProt P50990
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment CCT8 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8) (C-Term), (AA 519-548) antibody CCT8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCT8 antibody detected the CCT8 protein (arrow).
anti-Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8) (C-Term), (AA 519-548) antibody (2) CCT8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCT8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Chaperonin Containing TCP1, Subunit 8 (Theta) (CCT8) (C-Term), (AA 519-548) antibody (3) CCT8 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Background publications Zebol, Hewitt, Moretti et al.: "The CCT/TRiC chaperonin is required for maturation of sphingosine kinase 1." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 4, pp. 822-7, 2009 (PubMed).

Kim, Sprung, Chen et al.: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).

Wang, Zhu, Guo et al.: "A two-dimensional electrophoresis reference map of human ovary." in: Journal of molecular medicine (Berlin, Germany), Vol. 83, Issue 10, pp. 812-21, 2005 (PubMed).

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