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DSN1, MIND Kinetochore Complex Component, Homolog (S. Cerevisiae) (DSN1) (C-Term), (AA 257-286) antibody

Details for Product No. ABIN656747
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Antigen
Synonyms 1700022L09Rik, AW552447, C20orf172, KNL3, MIS13, dJ469A13.2, hKNL-3
Epitope
C-Term, AA 257-286
(10), (8), (2), (1), (1), (1), (1)
Reactivity
Human
(28), (11), (11), (11), (11)
Host
Rabbit
(27), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(18), (9), (9), (2), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN656747
291.50 $
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Immunogen This DSN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 AA from the C-terminal region of human DSN1.
Clone RB32490
Isotype Ig
Specificity This DSN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-295 amino acids from the C-terminal region of human DSN1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DSN1
Background This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants.
Synonyms: Kinetochore-associated protein DSN1 homolog,DSN1,C20orf172, MIS13
Molecular Weight 40067 DA
Gene ID 79980
UniProt Q9H410, NP_001138787.1, NP_079194.3, NP_001138788.1
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment DSN1 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-DSN1, MIND Kinetochore Complex Component, Homolog (S. Cerevisiae) (DSN1) (C-Term), (AA 257-286) antibody DSN1 Antibody (C-term) (ABIN656747) western blot analysis in MDA-MB231 cell line lysates (35 µg/lane). This demonstrates the DSN1 antibody detected the DSN1 protein (arrow).
Background publications Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Kiyomitsu, Obuse, Yanagida: "Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1." in: Developmental cell, Vol. 13, Issue 5, pp. 663-76, 2007 (PubMed).

Cheeseman, Desai: "Molecular architecture of the kinetochore-microtubule interface." in: Nature reviews. Molecular cell biology, Vol. 9, Issue 1, pp. 33-46, 2007 (PubMed).

Yang, Wu, Ward et al.: "Phosphorylation of HsMis13 by Aurora B kinase is essential for assembly of functional kinetochore." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26726-36, 2008 (PubMed).

Validation Images
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