tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 265-290), (Center), (Tyr284) antibody

Details for Product No. ABIN657857
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Antigen
Epitope
AA 265-290, Center, Tyr284
(23), (22), (12), (12), (10), (8), (7), (7), (7), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(141), (76), (63), (49), (49), (48), (12), (2)
Host
Rabbit
(139), (3), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB)
(89), (64), (40), (21), (21), (18), (12), (6), (5), (3), (3)
Pubmed 3 references available
Quantity 400 µL
Options
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Catalog No. ABIN657857
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Immunogen This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-290 AA from the Central region of human ACK1.
Clone RB10083
Isotype Ig
Specificity This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human ACK1.
Predicted Reactivity Cow (Bovine),Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ACK1
Background This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Synonyms: Activated CDC42 kinase 1,10188,ACK1
Molecular Weight 114569 DA
Gene ID 10188
NCBI Accession NP_001010938, NP_005772
UniProt Q07912
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin, Signaling
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment ACK1 Antibody (Center Y284) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 265-290), (Center), (Tyr284) antibody ACK1 Antibody (Center Y284) (ABIN657857) western blot analysis in NCI-H460 cell line lysates (35 µg/lane). This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).
Background publications Prieto-Echagüe, Gucwa, Craddock et al.: "Cancer-associated mutations activate the nonreceptor tyrosine kinase Ack1." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10605-15, 2010 (PubMed).

Chua, Lim, Tham et al.: "Somatic mutation in the ACK1 ubiquitin association domain enhances oncogenic signaling through EGFR regulation in renal cancer derived cells." in: Molecular oncology, Vol. 4, Issue 4, pp. 323-34, 2010 (PubMed).

Mahajan, Mahajan: "Shepherding AKT and androgen receptor by Ack1 tyrosine kinase." in: Journal of cellular physiology, Vol. 224, Issue 2, pp. 327-33, 2010 (PubMed).

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