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Details for Product No. ABIN93911

Tubulin, Beta, 3 (TUBB3) (N-Term) antibody

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Antigen
Synonyms CDCBM, CFEOM3A, TUBB4, beta-4, M(beta)3, M(beta)6, 3200002H15Rik, TUBB3, tbb3, tubb3, tubB3, tubulin
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Various Species
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Pubmed 12 references available
Catalog no. ABIN93911
Quantity 0.1 mg
Price
348.48 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 4 to 5 Business Days
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) ofneuron-specific human betaIII-tubulin.
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name betaIII-tubulin
Background The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one ofthe earliest markers of neuronal differentiation. It is regarded as a specific probe for the cellsof neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulinis most abundant in cells of neuronal origin, but was also detected in Sertoli cells of thetestis and transiently in non-neuronal embryonic tissues.
Application Notes Western Blotting - Recommended dilution: 1-2 µg/ml, 90 min Positive control: Porcine brain lysate. Negative control: HPB-ALL human peripheral blood leukemia cell line. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+)Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue. Immunocytochemistry - Positive material: Neuro2a mouse neuroblastoma cell line
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C
Storage Comment Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).

Kukharskyy, Sulimenko, Macůrek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).

Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).

Pĕknicová, Kubátová, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).

Woltjen, Michael, Mohseni et al.: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).

Silva, Pereira, Oliveira et al.: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).

Theodorou, Dalembert, Heffelfinger et al.: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).

Kunova, Matulka, Eiselleova et al.: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).

Alternatives for antigen "Tubulin, Beta, 3 (TUBB3)", type "Antibodies"
Hosts (73), (70), (6), (3)
Reactivities (124), (74), (70), (12), (10), (7), (5), (3), (2), (2), (2), (1), (1), (1)
Applications (113), (47), (45), (39), (36), (30), (27), (25), (17), (7), (4), (1), (1), (1)
Conjugates (12), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Epitopes (15), (13), (12), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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