Tubulin, Beta, 3 (TUBB3) (N-Term) antibody

Details for Product No. ABIN93911
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms tubb3, TUBB3, TUBB4, CDCBM, CFEOM3A, beta-4, 3200002H15Rik, M(beta)3, M(beta)6, AI325297, M(beta)4, Tubb, Tubb4
Epitope
N-Term
(17), (16), (13), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity
General
(146), (74), (69), (12), (6), (5), (4), (2), (1), (1)
Host
Mouse
(91), (64), (6), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Western Blotting (WB)
(123), (50), (43), (39), (39), (30), (27), (25), (15), (11), (1), (1)
Pubmed 12 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 4 to 5 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN93911
348.48 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Purification Purified by ammonium sulphate and caprylic acid precipitation
Purity > 95 % (by SDS-PAGE)
Alternative Name betaIII-tubulin
Background The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl,
Incubation 30 min in RT, or High temperature citrate buffer antigen retrieval
Positive tissue: neuronal tissueImmunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-Tubulin, Beta, 3 (TUBB3) (N-Term) antibody Immunofluorescence staining (mouse neuroblastoma cells)
Product cited in: Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).

Kukharskyy, Sulimenko, Macůrek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).

Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).

General Pĕknicová, Kubátová, Sulimenko et al.: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).

Woltjen, Michael, Mohseni et al.: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).

Silva, Pereira, Oliveira et al.: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).

Theodorou, Dalembert, Heffelfinger et al.: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).

Kunova, Matulka, Eiselleova et al.: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).

Validation Images
Did you look for something else?
back to top