CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (MEM-57)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN94058

Additional Information

Alternative name: CD3

Gene ID: 11q23

Immunogen: Human thymocytes and T lymphocytes.

Isotype: IgG2a

Clone: MEM-57

Description: CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of Tcells and in regulating the cell surface expression of the TCR complex. T cell activationthrough the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally relatedmembers of the immunoglobulins super family encoded by closely linked genes on humanchromosome 11. The CD3 components have long cytoplasmic tails that associate withcytoplasmic signal transduction molecules. This association is mediated at least in part by adouble tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play arole in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% ofthymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells.Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Specificity: The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed onperipheral blood T lymphocytes and mature thymocytes.HLDA IV., WS Code T 96

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 2 - 5 µg/ml. Positive control: Peripheral Blood LymphocytesJURKAT human leukemia T cell lineImmunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinatedT cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical patternyielded by a reference antibody Leu-4 (SK7). Functional Application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

Publications

Horejsí, Angelisová, Bazil et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74)." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988 (PubMed).

Hilgert, Franĕk, Stefanová et al.: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993 (PubMed).

Dave, Cao, Browne et al.: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997 (PubMed).

Panyi, Bagdány, Bodnár et al.: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Houben, Voigt, Noelke et al.: "MAPK-independent impairment of T-cell responses by the multikinase inhibitor sorafenib." in: Molecular cancer therapeutics, Vol. 8, Issue 2, pp. 433-40, 2009 (PubMed).

Paster, Paar, Eckerstorfer et al.: "Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2160-7, 2009 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Todaro, DAsaro, Caccamo et al.: "Efficient killing of human colon cancer stem cells by gammadelta T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 7287-96, 2009 (PubMed).

Muhammad, Schiller, Forster et al.: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

Mookerjee-Basu, Vantourout, Martinez et al.: "F1-Adenosine Triphosphatase Displays Properties Characteristic of an Antigen Presentation Molecule for V{gamma}9V{delta}2 T Cells." in: Journal of immunology (Baltimore, Md. : 1950), 2010 (PubMed).