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CD6 antibody (CD6)

Details for Product anti-CD6 Antibody No. ABIN94206, Supplier: Login to see
Antigen
  • TP120
  • OX52
Reactivity
Human
172
42
38
8
5
Host
Mouse
175
50
12
Clonality (Clone)
Monoclonal ()
Conjugate
This CD6 antibody is un-conjugated
27
27
16
4
3
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
162
94
66
50
35
32
29
25
11
7
6
5
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Immunogen Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).
Clone MEM-98
Isotype IgG1
Specificity The MEM-98 antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD6 (CD6 Antibody Abstract)
Background CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry: Recommended dilution: 2 µg/mL
Western Blotting:
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Positive tissue: spleen

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD6 antibody (CD6) (ABIN94206) Surface staining of human peripheral blood cells with anti-human CD6 (MEM-98) FITC.
Product cited in: Castro, Oliveira, Nunes et al.: "Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4351-61, 2007 (PubMed).

Hassan, Simmonds, Clarkson et al.: "CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6727-38, 2006 (PubMed).

Bazil, Stefanová, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Background publications Zimmerman, Joosten, Torensma et al.: "Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells." in: Blood, Vol. 107, Issue 8, pp. 3212-20, 2006 (PubMed).

Osorio, De Santiago, Aguilar-Santelises et al.: "CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM." in: Blood, Vol. 89, Issue 8, pp. 2833-41, 1997 (PubMed).